Effect of HGF on the Production of TGF-beta, Fibronectin, and Collagen Ia in the Murine Mesangial Cells.
- Author:
Won Suk AN
1
;
Hae Rahn BAE
;
Seo Hee RHA
;
Seong Eun KIM
;
Ki Hyun KIM
Author Information
1. Department of Internal Medicine, Dong-A University College of Medicine, Busan, Korea. kimkh@daunet.donga.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Hepatocyte growth factor;
Transforming growth factor;
Fibronectin;
Collagen Ia
- MeSH:
Animals;
Blotting, Western;
Collagen*;
Enzyme-Linked Immunosorbent Assay;
Fibronectins*;
Fibrosis;
Glucose;
Hepatocyte Growth Factor;
Injections, Subcutaneous;
Mesangial Cells*;
Mice;
Real-Time Polymerase Chain Reaction;
Regeneration;
RNA, Messenger;
Transforming Growth Factor beta*;
Transforming Growth Factors
- From:Korean Journal of Nephrology
2004;23(6):888-897
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Hepatocyte growth factor (HGF) is a multifunctional protein especially implicated in tissue repair and regeneration. In a murine model of chronic glomerulonepritis, subcutaneous injection of recombinant HGF inhibited renal fibrosis. Although these favorable results were accompanied by decreased expression of TGF-beta in renal tissue, the action of HGF on TGF-beta remains unclear. We studied whether HGF has an inhibitory effect on TGF-beta production in vitro. METHODS: Mouse mesangial cells grown in 5 mM glucose containing DMEM (NG, normal glucose) were exposed with 25 mM glucose medium (HG, high glucose) alone or in the presence of recombinant HGF (10 ng/mL) for 48 hours. TGF-beta in culture supernatants were measured by sandwich enzyme-linked immunosorbent assay. Secretion of fibronectin and collagen Ia was measured by western blotting and immunoprecipitaiton. TGF-beta, fibronectin and collagen Ia mRNA levels were measured by real-time PCR and internally corrected with GAPDH. RESULTS: High glucose significantly increased TGF-beta secretion (p<0.05), but HGF could not prevent high glucose-induced TGF-beta release. High glucose increased the secretion of both fibronectin and collagen Ia, which was blocked by HGF (p<0.05). CONCLUSION: In cultured murine mesangial cells HGF did not have effect on TGF-beta production, but inhibited the secretion of both fibronectin and collagen Ia. These results suggest that HGF may have indirect inhibitory effect on the action of TGF-beta.
