The Relationship between Insulin-like Growth Factor Binding Protein-3 Promoter Polymorphism and Growth.
- Author:
Woo Jin CHOI
1
;
Seung YANG
;
Jee Hye IM
;
Young Ik LEE
;
Jeh Hoon SHIN
Author Information
1. Department of Pediatrics, College of Medicine, Hallym University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
IGFBP-3;
Promoter;
Polymorphism;
Growth
- MeSH:
Alleles;
Body Height;
Child;
Gene Frequency;
Genotype;
Humans;
Insulin-Like Growth Factor Binding Protein 3;
Insulin-Like Growth Factor Binding Proteins;
Insulin-Like Growth Factor I;
Polymorphism, Restriction Fragment Length;
Polymorphism, Single Nucleotide;
Promoter Regions, Genetic
- From:Journal of Korean Society of Pediatric Endocrinology
2006;11(1):28-32
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The variability in insulin-like growth factor binding protein-3 (IGFBP-3) levels is related to polymorphic variants of the promoter region of the gene. The most common single nucleotide polymorphism of IGFBP promoter region is -202 locus. In vitro, significantly higher promoter activity of the A allele at the -202 locus compared with the C allele, consistent with the relationship observed between genotype and circulating IGFBP-3 was documented. The polymorphic variation occurs frequently and may influence GH responsiveness, somatic growth, but the effects of IGFBP-3 promoter polymorphism on growth in children are unknown. METHODS: RFLP genotyping of the -202 single nucleotide polymorphism was performed in 33 Korean children who visited our clinic for evaluation of their growth. The serum levels of IGF-I and IGFBP-3 were compared according to genotyping. RESULTS: Height SDS of subjects was -1.48+/-1.10. 11 of 33 children (33%) were heterozygous AC and the others are homozygous AA in genetic distribution, but no homozygous CC was detected. C allele frequency was 16.7%. There were no significant differences in height, body mass index, serum IGF-I and IGFBP-3 levels between AC and AA groups. CONCLUSION: The effects of IGFBP-3 promoter polymorphism on growth at the -202 locus deserve investigation because there is no CC and the children were relatively short in this study.
