Establishment and characterization of secondary drug resistant cell line originating from cisplatin-resistant SNU-8.
- Author:
Chun San AN
1
;
Sung Yob KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Cheju National University, Cheju, Korea. kimsy@cheju.ac.kr
- Publication Type:Original Article
- Keywords:
Ovarian cancer;
Multidrug resistance;
P-gp/MDR1;
ATP7B;
Cisplatin
- MeSH:
Blotting, Western;
Cell Line*;
Cisplatin;
Drug Resistance, Multiple;
Drug Therapy;
Humans;
Ovarian Neoplasms;
P-Glycoprotein;
RNA, Messenger
- From:Korean Journal of Obstetrics and Gynecology
2007;50(1):101-110
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Multidrug resistance to chemotherapy is a major obstacle in attempts to improve the clinical outcome of ovarian carcinoma patients. The aim of this study is to establish secondary anticancer drug resistant cell line from original SNU-8/WT resistant to cisplatin and characterize it. METHODS: After establishing secondary drug resistant cell line (SNU-8/Fac), drug sensitivity was measured by MTT assay. Multidrug resistance (MDR) was analyzed by RT-PCR and western blotting analysis. RESULTS: MTT assay data demonstrated that MDR was expressed in SNU-8/Fac. In addition, mRNA expression of MDR1, ATP7B in SNU-8/Fac was increased. However, overexpression of MRP1, BCRP, TS and MT mRNA was not observed. At the protein level, protein P-gp, ATP7B were overexpressed in SNU-8/Fac. CONCLUSION: We established a new anticancer drug resistant cell line from original SNU-8/WT. Overexpression of P-gp and ATP7B was observed in SNU-8/Fac.
