The expression and interaction of HLA-G and IL-10 in cervical cancer.
- Author:
Young Tae KIM
1
;
Bo Sung YOON
;
Jae Wook KIM
;
Sung Hoon KIM
;
Jae Hoon KIM
;
Sang Wun KIM
;
Eun Ji NAM
Author Information
1. Department of Obstetrics and Gynecology, Women's Cancer Clinic, Yonsei University College of Medicine, Seoul, Korea. ytkchoi@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
human leukocyte antigen-G;
interleukin-10;
cervical cancer
- MeSH:
Blotting, Western;
Carcinogenesis;
Cervix Uteri;
Female;
HLA-G Antigens*;
Humans;
Interleukin-10*;
RNA, Messenger;
Uterine Cervical Neoplasms*
- From:Korean Journal of Obstetrics and Gynecology
2007;50(1):126-134
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To determine the expression of HLA-G and IL-10 and their correlation in tissue of cervical cancer, and to investigate the relationship between their expression and clinicopathologic factors in patients with cervical cancer. METHODS: Tissue samples were obtained from 40 patients diagnosed with cervical cancer and 15 patients with normal cervix for control from Oct. 2004 to Oct. 2005. Quantitative real-time RT-PCR for HLA-G mRNA and semi-quantitative RT-PCR for IL-10 mRNA were used. And proteins of HLA-G and IL-10 were detected by Western blot analysis. RESULTS: Both HLA-G and IL-10 mRNA expression in cervical cancer tissue were higher than normal control, which was statistically significant (P<0.001, P<0.001). The proteins levels of HLA-G and IL-10 in cancer group were also significantly higher than control (P<0.001, P=0.041). The mRNA expression of HLA-G tended to be correlated with IL-10 expression (P=0.061), although it was not statistically significant. Among clinicopathologic factors of cervical cancer, there was inverse relationship between FIGO stage and mRNA value of HLA-G (P=0.045). CONCLUSIONS: The mRNA and protein expression of HLA-G and IL-10 in cervical cancer were much higher than in controls. These results showed that HLA-G and IL-10 might have an important role of tumorigenesis in patients with cervical cancer. The levels of HLA-G and IL-10 seem to be correlated although it was not statistically significant. High HLA-G mRNA expression could be related in early tumorigenesis since it was associated with early stage cervical cancer.
