The effects of chromium exposure on sister chromatid exchange and concentration of 8-hydroxydeoxyguanosine.
- Author:
Sang Hwan HAN
;
Soo Hun CHO
;
Heon KIM
;
Soo Min PARK
;
Mina HA
;
Young Soo JOO
;
Ho Jang KWON
;
Yong Dae KWON
;
Myung Hee KWON
- Publication Type:In Vitro ; Original Article
- Keywords:
chromate pigment;
8-hydroxydeoxyguanosine;
sister chromatid exchange
- MeSH:
Biomarkers;
Chromates;
Chromatids;
Chromatography, Liquid;
Chromium*;
Creatinine;
DNA;
DNA Adducts;
Environmental Exposure;
Gyeonggi-do;
Humans;
Lymphocytes;
Molar;
Plants;
Siblings*;
Sister Chromatid Exchange*;
Smoke;
Smoking
- From:Korean Journal of Preventive Medicine
1995;28(2):511-525
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To elucidate some DNA adducts as a biological marker for workers of chromate pigment, the effects of chromium exposure on the formation of 8-hydroxydeoxyguanosine(8-OH-dG) and sister chromatid exchanges(SCEs) frequency in 38 workers of a pigment plant in Bucheon which utilized lead chromates, were examined. The chromium contents of venous blood and urine were measured as working environmental exposure level. The concentrations of 8-OH-dG in DNA isolated from lymphocytes were determined with high performance liquid chromatography and electrochemical detector and denoted as a molar ratio of 8-OH-dG to deoxyguanosine(dG). The SCEs frequency were analyzed in DNA isolated from lymphocytes. A significant correlation was found between creatinine adjusted urine chromium concentration and the molar ratio of 8-OH-dG to dG(r=0.47, p<0.01). After adjusting the current smoking habit, the correlation coefficient was increased(r=0.62, p<0.05). However, there was no significant correlation between the SCE frequency and chromium exposure. This significant results between molar ratio of 8-OH-dG to dG and chromium exposure are in good agreement with in vitro studies that support the importance of DNA adduct formation for the carcinogenic effect of chromium.
