Relationship between Mitochondrial DNA Point Mutation and Noise-induced Sensorineural Hearing Loss.
- Author:
Sung Hee PARK
1
;
Tae Wan KIM
;
Mi Young LEE
;
Jae Hoon BAE
;
Dong Hoon SHIN
Author Information
1. Department of Preventive Medicine, Keimyung University School of Medicine, Daegu, Korea. dhshin@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Mitochondrial DNA mutation;
Noise-induced sensorineural hearing loss
- MeSH:
Clinical Coding;
Deafness;
DNA;
DNA Restriction Enzymes;
DNA, Mitochondrial*;
Genes, rRNA;
Genome, Mitochondrial;
Hearing Loss;
Hearing Loss, Noise-Induced;
Hearing Loss, Sensorineural*;
Humans;
Medical Records;
Point Mutation*;
Polymerase Chain Reaction;
Sequence Analysis, DNA
- From:Korean Journal of Aerospace and Environmental Medicine
2005;15(3):93-99
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: A different sequence change in the mitochondrial 12S rRNA gene has been proposed as a candidate mutation in the sensorineural hearing loss. The purpose of this study was to study the association between the noise-induced sensorineural hearing loss and A to G mutation at nucleotide 1555 of mitochondrial DNA. METHODS: subjects were reviewed by history and medical records, and audiological and clinical data were obtained. Blood sampling was done on 101 normal controls, 51 with noise-induced sensorineural hearing loss, and 12 with sensorineurnal deafness. The DNA of these individuals were extracted, and mitochondrial genome were analyzed by polymerase chain reaction (PCR). Subsequently, the coding sequence of mitochondrial genome was sequenced and compared to the normal sequence, and all sequence variations were analyzed by restriction endonuclease BsmA I. RESULTS: Mitochondrial DNA mutant (1555A-->G) was not detected by PCR in all Korean patients with noise-induced hearing loss, sensorineural hearing loss, and in normal controls with no hearing loss. The DNA sequencing of PCR products did not reveal an A to G substitution at nucleotide 1,555 of mitochondrial DNA. CONCLUSION: This result suggests that the noise-induced sensorineural hearing loss is not associated with mitochondrial DNA mutation (1555A-->G).
