Mechanism of VanB Phenotype in Vancomycin-Resistant Enterococci carrying vanA gene.
10.3343/kjlm.2006.26.6.412
- Author:
Il Joong PARK
1
;
Wee Gyo LEE
;
Hyukmin LEE
;
Dongeun YONG
;
Kyungwon LEE
;
Eui Chong KIM
;
Seok Hoon JEONG
;
Yeon Jun PARK
;
Tae Yeal CHOI
;
Young UH
;
Jong Hee SHIN
;
Jongwook LEE
;
Ji young AHN
;
Sung Hee LEE
;
Gun Jo WOO
Author Information
1. Department of Family Medicine, Ajou University School of Medicine, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
VRE;
VanB;
vanA;
vanS;
vanX;
vanY;
vanZ
- MeSH:
DNA;
Enterococcus faecium;
Genes, Regulator;
Genotype;
Hospitals, University;
Humans;
Japan;
Korea;
Nucleotides;
Phenotype*;
Point Mutation;
Polymerase Chain Reaction;
Research Personnel;
Taiwan;
Teicoplanin
- From:The Korean Journal of Laboratory Medicine
2006;26(6):412-417
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Recently, vancomycin-resistant enterococci (VRE) with the vanA genotype that are susceptible to teicoplanin have been described in Japan, Taiwan, and Korea. The investigators suggested three point mutations in the putative sensor domain of vanS or impairment of accessory proteins VanY and VanZ as an explanation for the VanB phenotype-vanA genotype VRE. In this study, we analyzed Tn1546-like elements to determine the molecular mechanisms responsible for the impaired glycopeptide resistance of clinical VRE isolates with VanB phenotype-vanA genotype from Korea. METHODS: From 2001 to 2004, 28 clinical isolates of Enterococcus faecium with VanB phenotypevanA genotype were collected from 8 different university hospitals in diverse geographic areas in Korea. For structural analysis of Tn1546-like elements, PCR amplifications for internal regions of Tn1546 were performed. The purified PCR products were directly sequenced with an ABI Prism 3100 DNA sequencer. RESULTS: The sequence data of the vanS regulatory gene revealed that none of the isolates had any point mutations in this gene. All 28 isolates had a complete or incomplete deletion of vanY gene. Of these, 13 strains represented a complete deletion of vanZ, and 2 strains showed the deletion of nucleotides near the end point of vanX. CONCLUSIONS: The mechanism of VanB phenotype-vanA genotype in VRE isolates from Korea is not point mutations of vanS but the rearrangements of vanX, vanY and vanZ.
