Isolation and identification of canine parvovirus type 2b in Korean dogs.
10.14405/kjvr.2015.55.3.163
- Author:
Dong Kun YANG
1
;
Kyung Suk KANG
;
Hyun Ye JO
;
Ha Hyun KIM
;
Sung Suk CHOI
;
Jae Young SONG
Author Information
1. Viral disease division, Animal and Plant Quarantine Agency, Anyang 430-757, Korea. yangdk@korea.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
CPV-2b;
canine parvoviral enteritis;
virus isolation
- MeSH:
Amino Acid Sequence;
Amino Acids;
Animals;
Asian Continental Ancestry Group;
Base Sequence;
Dogs*;
Fluorescence;
Gyeonggi-do;
Humans;
Korea;
Parvovirus, Canine*
- From:Korean Journal of Veterinary Research
2015;55(3):163-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
Canine parvovirus (CPV) is a major diarrhea-causing agent in puppies. Since CPV type 2 (CPV-2) emerged in 1978, new antigenic variants including CPV-2a, CPV-2b, and CPV-2c have been identified in many countries. Two puppies died suddenly at a veterinary clinic in Gyeonggi province, South Korea. Two viruses were isolated in A72 cells, confirmed as CPV strains based on a CPV rapid kit and an indirect fluorescence test and designated QIACP1403 and QIACP1404. The nucleotide sequences of complete VP2 genes of QIACP1403 and QIACP1404 were determined, and the corresponding amino acid sequences were deduced. Molecular analyses revealed that the QIACP1403 and QIACP1404 isolates were type CPV-2b. Several mutated amino acids were detected on VP2 gene residues of the two isolates. Phylogenetic analyses showed that the two isolates were most closely related to strain CPV-BM11, which was isolated from Chinese dogs in 2011. Our results suggest that these isolates may be a candidate for a vaccine to prevent CPV infection in dogs after conducting passages of the isolates in an in vitro culture system.
