Eupatilin Ameliorates Collagen Induced Arthritis.
10.3346/jkms.2015.30.3.233
- Author:
Juryun KIM
1
;
Youngkyun KIM
;
Hyoju YI
;
Hyerin JUNG
;
Yeri Alice RIM
;
Narae PARK
;
Seung Min JUNG
;
Sung Hwan PARK
;
Ji Hyeon JU
Author Information
1. Clinical Immunology and STEM (CiSTEM) Cell Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. juji@catholic.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Eupatilin;
Arthritis, Experomental;
Arthritis, Rheumatoid;
DA-9601
- MeSH:
Animals;
Anti-Inflammatory Agents/pharmacology/*therapeutic use;
Arthritis, Experimental/chemically induced/*drug therapy;
Arthritis, Rheumatoid/drug therapy/pathology;
Cell Differentiation/*drug effects;
Cells, Cultured;
Collagen Type II;
Cytokines/biosynthesis;
Disease Models, Animal;
Drugs, Chinese Herbal/therapeutic use;
Female;
Flavonoids/pharmacology/*therapeutic use;
Humans;
Inflammation/drug therapy/immunology;
Interleukin-1beta/genetics/metabolism;
Interleukin-6/genetics/metabolism;
Lymph Nodes/cytology;
Mice;
Mice, Inbred DBA;
Monocytes/cytology;
Osteoclasts/*cytology;
Plant Extracts/pharmacology;
RNA, Messenger/biosynthesis;
Synovial Membrane/cytology;
T-Lymphocytes, Regulatory/cytology/immunology;
Tumor Necrosis Factor-alpha/pharmacology
- From:Journal of Korean Medical Science
2015;30(3):233-239
- CountryRepublic of Korea
- Language:English
-
Abstract:
Eupatilin is the main active component of DA-9601, an extract from Artemisia. Recently, eupatilin was reported to have anti-inflammatory properties. We investigated the anti-arthritic effect of eupatilin in a murine arthritis model and human rheumatoid synoviocytes. DA-9601 was injected into collagen-induced arthritis (CIA) mice. Arthritis score was regularly evaluated. Mouse monocytes were differentiated into osteoclasts when eupatilin was added simultaneously. Osteoclasts were stained with tartrate-resistant acid phosphatase and then manually counted. Rheumatoid synoviocytes were stimulated with TNF-alpha and then treated with eupatilin, and the levels of IL-6 and IL-1beta mRNA expression in synoviocytes were measured by RT-PCR. Intraperitoneal injection of DA-9601 reduced arthritis scores in CIA mice. TNF-alpha treatment of synoviocytes increased the expression of IL-6 and IL-1beta mRNAs, which was inhibited by eupatilin. Eupatilin decreased the number of osteoclasts in a concentration dependent manner. These findings, showing that eupatilin and DA-9601 inhibited the expression of inflammatory cytokines and the differentiation of osteoclasts, suggest that eupatilin and DA-9601 is a candidate anti-inflammatory agent.
