DNA Methylation and Expression Patterns of Key Tissue-specific Genes in Adult Stem Cells and Stomach Tissues.
10.3346/jkms.2009.24.5.918
- Author:
Seung Jin HONG
1
;
Moo Il KANG
;
Jung Hwan OH
;
Yu Chae JUNG
;
Young Ho KIM
;
Sung Ja KIM
;
Seung Hye CHOI
;
Eun Joo SEO
;
Sang Wook CHOI
;
Mun Gan RHYU
Author Information
1. Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Korea. rhyumung@catholic.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
DNA Methylation;
Stem Cells;
Stomach;
Tissue-Specific Gene;
CpG Islands
- MeSH:
Adipose Tissue/cytology;
Adolescent;
Adult;
Adult Stem Cells/cytology/*metabolism;
Aged;
CpG Islands/*genetics;
*DNA Methylation;
Female;
Gene Expression Profiling;
Humans;
Male;
Middle Aged;
Polymerase Chain Reaction;
Stomach/cytology/*metabolism;
Stromal Cells/metabolism;
Transcription Initiation Site;
Transcription, Genetic
- From:Journal of Korean Medical Science
2009;24(5):918-929
- CountryRepublic of Korea
- Language:English
-
Abstract:
CpG-island margins and non-island-CpG sites round the transcription start sites of CpG-island-positive and -negative genes are methylated to various degrees in a tissue-specific manner. These methylation-variable CpG sites were analyzed to delineate a relationship between the methylation and transcription of the tissue-specific genes. The level of tissue-specific transcription was estimated by counting the number of the total transcripts in the SAGE (serial analysis of gene expression) database. The methylation status of 12 CpG-island margins and 21 non-island CpG sites near the key tissue-specific genes was examined in pluripotent stromal cells obtained from fat and bone marrow samples as well as in lineage-committed cells from marrow bulk, stomach, colon, breast, and thyroid samples. Of the 33 CpG sites examined, 10 non-island-CpG sites, but none of the CpG-island margins were undermethylated concurrent with tissue-specific expression of their nearby genes. The net methylation of the 33 CpG sites and the net amount of non-island-CpG gene transcripts were high in stomach tissues and low in stromal cells. The present findings suggest that the methylation of the non-island-CpG sites is inversely associated with the expression of the nearby genes, and the concert effect of transitional-CpG methylation is linearly associated with the stomach-specific genes lacking CpG-islands.
