Altered Vascular Expression of Nitric Oxide Synthase Isozymes in Hypertension.
- Author:
In Kwang KIM
1
;
Dae Gil KANG
;
Jong Eun LEE
;
Bong Suk OH
Author Information
1. Department of Thoracic and Cardiovascular Surgery, Chonnam University Medical School.
- Publication Type:Original Article
- Keywords:
Nitric oxide;
Hypertension
- MeSH:
Animals;
Aorta, Thoracic;
Arterial Pressure;
Blotting, Western;
Desoxycorticosterone;
Hypertension*;
Isoenzymes;
Nitric Oxide Synthase*;
Nitric Oxide*;
Nitrites;
Protein Isoforms;
Rats;
Vasodilation
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
1999;32(2):138-143
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The endothelium-dependent vasorelaxation has been largely accounted for by the release of nitric oxide (NO). Three distinct isoforms of NO synthases (NOS) have been characterized, i.e., brain(bNOS), inducible (iNOS), and endothelial constitutive (ecNOS). Although hypertension hasbeen associated with a vascular endothelial dysfunction, changes in the vascular expression of NOS isoforms have not been established. The present study was aimed at exploring the vascular expression of NOS isozymes in hypertension. MATERIAL AND METHOD: Two-kidney, one clip (2K1C) and deoxycorticosterone acetate (DOCA)-salt hypertension were induced in rats. The expression of different NOS isozymes in the thoracic aorta was determined by Western blot analysis. The vascular tissue contents of nitrites were measured by colorimetric assay. RESULT: Arterial blood pressure was significantly higher in experimental groups of 2K1C and DOCA-salt rats compared with their corresponding control rats. The vascular expression of bNOS as well as that of ecNOS was decreased in both models of hypertension. iNOS was not changed in DOCA-salt hypertension, but was also decreased in 2K1C hypertension. The vascular contents of nitrites were significantly decreased in DOCA-salt as well as in 2K1C hypertension. CONCLUSION: These results suggest that 2K1C and DOCA-salt hypertension are associated with decreases in the vascular expression of NOS isozymes and nitrite contents.
