In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks.
- Author:
Kyu Ho KIM
1
;
Suk Jin CHOI
;
Yeon Il CHOI
;
Lucia KIM
;
In Suh PARK
;
Jee Young HAN
;
Joon Mee KIM
;
Young Chae CHU
Author Information
1. Department of Pathology, Inha University Hospital, Inha University College of Medicine, Incheon, Korea. sukjinchoi007@gmail.com
- Publication Type:Original Article
- Keywords:
Tissue array analysis;
Sepharose;
Paraffin;
Pathology;
Research
- MeSH:
Gels;
Humans;
Paraffin;
Sepharose;
Tissue Array Analysis;
Tissue Donors
- From:Korean Journal of Pathology
2013;47(3):238-244
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Self-made tissue punches can be effectively used to punch holes in blank recipient paraffin blocks and extract tissue cores from the donor paraffin blocks for the low-cost construction of tissue microarrays (TMAs). However, variable degrees of section distortion and loss of the tissue cores can occurs during cutting of the TMAs, posing technical problems for in-house manual construction of high-density TMAs. We aimed to update the method for in-house manual TMA construction to improve the quality of high-density TMAs. METHODS: Blocks of agarose gel were subjected to the standard tissue processing and embedding procedure to prepare recipient agarose-paraffin blocks. The self-made tissue punches and recipient agarose-paraffin blocks were used to construct TMAs, which were completely melted and re-embedded in paraffin to make finished TMA blocks. RESULTS: The donor tissue cores were completely integrated into the surrounding paraffin of the recipient blocks. This method enabled us to construct high-density TMAs with significantly less section distortion or loss of tissue cores during microtomy. CONCLUSIONS: Simple and inexpensive construction of high-density and high-quality TMAs can be warranted by using paraffinized agarose gels as recipient blocks.
