Detection of Platelet-Specific Antibodies Employing Modified Antigen Capture ELISA(MACE).
- Author:
Tae Hyun UM
;
Kyou Sup HAN
;
Dae Chul KIM
;
Yoo Sung HWANG
;
Doo Sung KIM
;
Sang In KIM
- Publication Type:Original Article
- MeSH:
Antibodies*;
Antibodies, Monoclonal;
Blood Platelets;
Fluorescent Antibody Technique;
Glycoproteins;
HLA Antigens;
Humans;
Immunoenzyme Techniques;
Isoantibodies;
Korea;
Male;
Tissue Donors
- From:Korean Journal of Blood Transfusion
1995;6(2):123-130
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Widely used tests for the detection of platelet antibodies in Korea include platelet suspension immunofluorescence test(PSIFT), enzyme immunoassay and mixed passive hemagglutination(MPHA). In these tests, removal of HLA antigens from platelet are required to detect platelet-specific antibodies. Modified antigen capture ELISA(MACE) is known to be very sensitive for the detection of platelet-specific antibodies, in which specific platelet glycoprotein, captured by the monoclonal antibody is used as a target antigen. MACE is very useful for the detection of platelet-specific alloantibodies in neonatal alloimmune thrombocytopenia(NAIT) and posttransfusion purpura(PTP). We employed MACE in our laboratory, using AP2(anti-GPIIb/IIIa, monoclonal), #30 sera(anti-PlA1), 90-545 sera(anti-HLA-B51+52) and LYS sera(multispecific HLA antibodies). LYS sera had been used as our positive control( 1:120) in MPHA. Platelet from PIA1(+), HLA-B5 I, blood group O healthy male donor, gave positive result with #30 sera(1:40) and negative result with 90-545 sera in MACE. With LYS sera, MACE showed negative in 1:120, but positive in 1:20. So LYS sera was thought to contain strong multispecific HLA antibodies and relatively weak antibody(-ies) reacting with GPllb/Illa. Further studies employing different monoclonal antibodies, such as anti-GPIb/IX, -GPIa/Ila and -GPIV are under way.
