Characteristics of Seizures and Neuronal Nitric Oxide Synthase Expression of Hippocampus in Hyperthermia-Induced Seizures of Developing Rat.
- Author:
Doo Kwun KIM
1
;
Dong Keun JUNG
Author Information
1. Department of Pediatrics, Dongguk University College of Medicine, Kyungju, Korea. pedepi@hotmail.com
- Publication Type:Original Article
- Keywords:
Hyperthermic seizures;
Neuronal nitric oxide synthase;
Spraque-Dowley rat
- MeSH:
Animals;
Baths;
Blotting, Western;
Fever;
Hippocampus*;
Neurons*;
Nitric Oxide Synthase Type I*;
Rats*;
Seizures*;
Water
- From:Journal of the Korean Pediatric Society
2002;45(5):629-636
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Our study was aimed to investigate the characteristics of seizures as well as to determine whether the expression of neuronal nitric oxide synthase expression(nNOS) of hippocampus has an affect in the hyperthermic seizure in developing rat. METHODS: Hyperthermic seizures were repeatedly induced twice a week for four weeks in 20-day old Spraque-Dowley rats. Fifty two rats were used as a hyperthermic group and 30 rats used as a normothermic control group. Hyperthermic seizures were induced in a water bath at 45degreesC+/-1 for 4 min. The characteristics of seizures were recorded. Using western blot, hippocampal nNOS expression was measured in normothermic control, hyperthermic non-seizure, and hyperthermic seizure groups, respectively. RESULTS:Eighty seven percent of hyperthermia exposed rats showed generalized tonic-clonic seizure most frequently. The duration of seizure was ranged from 12 to 145 sec(mean 55 sec) and the latency to seizure ranged from 158 to 240 sec(mean 204 sec). The duration of seizure was prolonged but there was no significant difference in the seizure latency as the rat exposed more number of hyperthermia. Interestingly, the expression level of hippocampal nNOS in hyperthermic seizure and hyperthermic non-seizure groups was not different from each other, however, the expression in these groups was lower than that of the control group. CONCLUSION: Our results indicate that nNOS do not have an affect in this repeated hyperthermic seizures. Further studies are required to clarify a role of nNOS in hyperthermic seizure.
