The Bone Grinding and Scaffold Grafting Techniques for Guide Bone Regeneration Induce the Stress on the Rat Brain.
10.5625/lar.2010.26.3.233
- Author:
Yeon Kyung LEE
1
;
Ji Eun KIM
;
So Hee NAM
;
Sun il CHOI
;
Eon Pil LEE
;
Hae Wook CHOI
;
Hong Sung KIM
;
Jae Ho LEE
;
Young Jin JUNG
;
Byeong Cheol KANG
;
Jung Sik CHO
;
Dae Youn HWANG
Author Information
1. Department of Biomaterials Science, College of Natural Resources & Life Science, Pusan National University/PNU-Laboratory Animal Resources Center, Miryang, Korea. dyhwang@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Guided bone regeneration;
cortisol;
mitogen-activated protein kinase (MAPK);
stress;
apatitte membrane
- MeSH:
Animals;
Blotting, Western;
Bone Regeneration;
Brain;
Connective Tissue;
Epithelium;
Hydrocortisone;
JNK Mitogen-Activated Protein Kinases;
Membranes;
Osteogenesis;
Phosphorylation;
Phosphotransferases;
Rats;
Skull;
Transplants
- From:Laboratory Animal Research
2010;26(3):233-239
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Guided bone regeneration (GBR) is a technique that a barrier membrane is placed over the bone defect to prevent the cell growth from the connective tissue and epithelium. In this study, in order to determine whether GBR technique could induce stress in rats, the standardized bone defect in rat calvaria was covered with apatitte membrane. Bone and brain tissues were collected from rats at 3 days, 2, 4, and 16 weeks post-operation, and then alteration of the new bone formation at the defects and stress-related factors were detected with histological examination and Western blot, respectively. From 4 to 16 weeks after the operation, the apatitte membrane was attached to the region of regenerated bone and encapsulated with a thick fibrous layer. Furthermore, the concentration of cortisol, a good indicator of stress, significantly increased 3 days post-operation. However, the increase at 3 days was returned to the basal level in 2 weeks. In Western blot analysis, the highest phosphorylation level of extracellular signal-regulated kinase (ERK) was observed 3 day post-operation, while those of the c-jun N-terminal kinase (JNK) and p38 were detected 4 weeks post-operation. Taken together, the results suggest that GBR technique may induce the serious stress on the brain tissue via the induction of ERK phosphorylation during 2 weeks, and that the stress responses restored in 4 week via JNK and p38 signaling pathway.
