Cultured Corneal Keratocyte: Scanning and Transmission Electron Microscopic Findings.
- Author:
Myung Kyoo KO
1
;
Dong Cheol KIM
Author Information
1. Department of Ophthalmology, School of Medicine, Hanyang University, Seoul, Korea.
- Publication Type:Original Article
- MeSH:
Cells, Cultured;
Corneal Keratocytes*;
Corneal Stroma;
Microdissection;
Serial Passage
- From:Journal of the Korean Ophthalmological Society
1987;28(2):279-284
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The cultured cells were derived from rabbit corneal stroma by explant technique following microdissection and serial passage. The ultrastructural features of fourteenth-passage keratocytes were examined with both scanning and transmission electron microscope. The cells can be divided into activated, intermediate and old cell according to the differences in electron density and surface microvillous pattern. The morphologic characteristics of the cultured keratocytes partially resemble those shown in corneal keratocytes in vivo.