Loquat (Eriobotrya japonica) extracts suppress the adhesion, migration and invasion of human breast cancer cell line.
- Author:
Min Sook KIM
1
;
Mi Kyoung YOU
;
Dong Young RHUY
;
Yung Jae KIM
;
Hum Young BAEK
;
Hyeon A KIM
Author Information
- Publication Type:Original Article
- Keywords: Loquat (Eriobotrya japonica); adhesion; migration; invasion; MMP
- MeSH: Breast Neoplasms; Cell Line; Dimethyl Sulfoxide; Eriobotrya; Humans; Methanol; Neoplasm Metastasis; Seeds
- From:Nutrition Research and Practice 2009;3(4):259-264
- CountryRepublic of Korea
- Language:English
- Abstract: We examined the inhibitory effects of loquat methanol extract on the adhesion, migration, invasion and matrix metalloproteinase (MMP) activities of MDA-MB-231 human breast cancer cell line. Cells were cultured with DMSO or with 10, 25, or 50 microg/ml of loquat methanol extract. Both leaf and seed extracts significantly inhibited growth of MDA-MB-231 cells in a dose-dependent manner, although leaf extract was more effective. Adhesion and migration were significantly inhibited by loquat extracts in a dose-dependent manner. Loquat extract also inhibited the invasion of breast cancer cells in a dose-dependent manner and leaf extract was more effective than seed extract. MMP-2 and MMP-9 activities were also inhibited by loquat extract. Our results indicate that methanol extracts of loquat inhibit the adhesion, migration and invasion of human breast cancer cells partially through the inhibition of MMP activity and leaf extract has more anti-metastatic effects in cell based assay than seed extract. Clinical application of loquat extract as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.
