Effect of Hydrogen Peroxide-induced Oxidative Stress on the Senescence of Trabecular Meshwork Cells.
10.3341/jkos.2008.49.10.1665
- Author:
Jae Woo KIM
1
;
Sin Hoo KIM
;
Jae Hyung LEE
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Koera. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Ascorbic acid;
Hydrogen peroxide;
Oxidative stress;
Senescence;
Trabecular meshwork cells
- MeSH:
Aging;
Ascorbic Acid;
Cell Aging;
Glaucoma;
Humans;
Hydrogen;
Hydrogen Peroxide;
Oxidative Stress;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
2008;49(10):1665-1670
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of oxidative stress on the senescence of trabecular meshwork (TM) cells and the effect of L-ascorbic acid (LAA) against oxidative stress-induced senescence. METHODS: Primary cultured human TM cells were exposed to 0.05 or 0.1 mM hydrogen peroxide for 30 minutes and incubated for 1 week with or without co-exposure of LAA. Cellular survival, nitrite production, and senescence were assessed with MTT, Griess, and SA-beta-gal assays, respectively. RESULTS: Hydrogen peroxide decreased cellular survival and NO production accompanied increased cellular senescence. LAA did not prevent hydrogen peroxide-induced senescence. CONCLUSIONS: Oxidative stress-induced senescence of TM cells may be related to the dysfunction of trabecular meshwork in glaucoma.
