Regulation of V-cadherin Expression on Human Dermal Microvascular Endothelial Cells.
- Author:
Yi Sun KIM
1
;
Hyun Joo CHOI
;
Min Geol LEE
;
Kwang Hoon LEE
Author Information
1. Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Dermal microvascular;
Endothelial cells;
V-cadherin
- MeSH:
Cadherins;
Cell Adhesion;
Endothelial Cells*;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Fluorescent Antibody Technique;
Glycoproteins;
Humans*;
Inflammation;
Interleukin-1
- From:Korean Journal of Dermatology
1997;35(5):924-930
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Cadherins are a supergene family of structurally related transmembrane glycoprotein. V-cadherin was a subclass of cadherin which expressed on endothelial cell as an endothelial cell cell adhesion molecule. The role of V-cadherin is still unclear in inflarnmation and immunologic responses. OBJECTIVE: Our purpose was to demonstrate the expression and the regulation of V-cadherin expression on human dermal microvascular endothelial cells(HDMEC) by biological response modifier(BRM). METHODS: The cell surface expression of V-cadherin on HDMEC that was unstimulated or pretreated with IL-1, TNF, IFN-, and PMA was evaluated by immunofluorescence flow cytometry and the enzyme-linked immunosorbent assay. RESULTS: 1. V-cadherin was constitutively expressed on unstimulated HDMEC. 2. Maximal expression of V-cadherin was observed at 16 hrs after stimulation with IL-1, at 6 hrs after stimulation with TNF and at 24 hrs after stimulation with IFN- on HDMEC. 3. V-cadherin expression on HDMEC was increased after stimulation with IL-1 or PMA. CONCLUSION: V-cadherin was constitutively expressed on HDMEC, and V-cadherin expression can be regulated by BRM. These results suggest V-cadherin may contribute to inflammation.
