Identification of differentially expressed genes using an annealing control primer system in periodontitis.
- Author:
Hee Sam NA
1
;
Ji S KIM
;
Jin CHUNG
Author Information
1. Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan, Korea. jchung@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
periodontitis ACP-PCR;
differentially expressed genes
- MeSH:
Humans;
Periodontitis;
Polymerase Chain Reaction
- From:International Journal of Oral Biology
2012;37(3):109-114
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In this study, we screened for differentially expressed genes (DEGs) in periodontitis compared with normal tissue using an annealing control primer (ACP) system. By ACP RT-PCR analysis, we obtained about 160 amplicons, 8 of which were found to be differentially expressed. DEGs in patients with periodontitis were thus successfully and reliably identified by the ACP-based RT PCR technique. The DEGs identified in the screen may also enhance our understanding of the pathogenesis of periodontitis.
