MicroRNA Analysis during Cultured Odontoblast Differentiation.
- Author:
Min Gyeong PARK
1
;
Myoung Hwa LEE
;
Sun Kyoung YU
;
Euteum PARK
;
Seog KIM
;
Seul Ah LEE
;
Yeon Hee MOON
;
Heung Joong KIM
;
Chun Sung KIM
;
Do Kyung KIM
Author Information
1. Oral Biology Research Institute, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea. kdk@chosun.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
miRNA;
odontoblast;
differentiation;
microarray
- MeSH:
Animals;
Base Pairing;
Cell Differentiation;
Dental Papilla;
Mice;
Microarray Analysis;
MicroRNAs;
Nucleotides;
Odontoblasts;
Protein Biosynthesis;
Real-Time Polymerase Chain Reaction;
RNA, Messenger
- From:International Journal of Oral Biology
2012;37(3):146-152
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
MicroRNAs (miRNAs, miRs) are about 21-25 nucleotides in length and regulate mRNA translation by base pairing to partially complementary sites, predominantly in the 3'-untranslated region (3'-UTR) of the target mRNA. In this study, the expression profile of miRNAs was compared and analyzed for the establishment of miRNA-related odontoblast differentiation using MDPC-23 cells derived from mouse dental papilla cells. To determine the expression profile of miRNAs during the differentiation of MDPC-23 cells, we employed miRNA microarray analysis, quantitative real-time PCR (qRT-PCR) and Alizaline red-S staining. In the miRNA microarray analysis, 11 miRNAs were found to be up- or down-regulated more than 3-fold between day 0 (control) and day 5 of MDPC-23 cell differentiation among the 1,769 miRNAs examined. In qRT-PCR analysis, the expression levels of two of these molecules, miR-194 and miR-126, were increased and decreased in the control MDPC-23 cells compared with the MDPC-23 cells at day 5 of differentiation, respectively. Importantly, the overexpression of miR-194 significantly accelerated mineralization compared with the control cultures during the differentiation of MDPC-23 cells. These results suggest that the miR-194 augments MDPC-23 cell differentiation, and potently accelerates the mineralization process. Moreover, these in vitro results show that different miRNAs are deregulated during the differentiation of MDPC-23 cells, suggesting the involvement of these genes in the differentiation and mineralization of odontoblasts.
