The Expression of Keratinocyte Growth Factor mRNA in Dendritic Epidermal T Cell.
- Author:
Kang Seok LEE
;
Seong Jun SEO
;
Myeung Nam KIM
;
Byung In RO
;
Chang Kwun HONG
- Publication Type:Original Article
- Keywords:
Dendritic epidermal T cell;
Keratinocyte growth factor
- MeSH:
Cytokines;
Epidermis;
Epithelial Cells;
Fibroblast Growth Factor 7*;
Fibroblasts;
Granulocyte-Macrophage Colony-Stimulating Factor;
Humans;
Interleukin-4;
Keratinocytes*;
RNA, Messenger*;
Skin;
T-Lymphocytes
- From:Korean Journal of Dermatology
1999;37(3):290-296
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The keratinocyte growth factor(KGF) is a recently identified mitogen for epithelial cells produced by nomal stromal fibroblasts. ln the skin, KGF has been shown to stimulate keratinocyte proliferation and differentiatian. Dendritic epidermal T cells(DETC) are skin-specific members of the epithelial y 8 T-cell family that reside normally in the murine epidermis. The DETCs recognize antigen expressed by damaged or diseased neighboring keratinoctyes and consequently secrete cytokines sueh as IFN- y, lL-2, IL-4, GM-CSF. OBJECTIVE: The purpose of this study was to observe the expression of KGF mRNA in keratinocyte and DETC as well as to investigate the cytokine-mediated intercellular communication between kerati- nocyte and DETC. METHODS: Using a RT-PCR(reverse transcription-polymerase chain reaction), we examined the expression of KGF mRNA in keratinocyte and DETC, and compared the level of KGF mRNA between resting and activated DETC with Con-A (concanavalin A).
