The Effects of Hydrogen Peroxide on the Migration and Proliferation of the Human Keratinocytes during Wound Healing.
- Author:
Hyang Joon PARK
;
Jeong Heon LEE
- Publication Type:In Vitro ; Original Article
- Keywords:
Hydrogen peroxide;
Wound healing;
keratinocyte migration & proliferation
- MeSH:
Humans*;
Hydrogen Peroxide*;
Hydrogen*;
Keratinocytes*;
Postoperative Care;
Thymidine;
Wound Healing*;
Wounds and Injuries*
- From:Korean Journal of Dermatology
1999;37(3):352-357
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Various topical antisepties are often used for postoperative care of surgical wounds. But the effect of these agent on wound healing remains an area of debate. OBJECTIVE: The purpose of this study was to evaluate the biological effects of hydrogen peroxide on human keratinocytes in vitro, specifically on keratinocyte viability, migration and proliferation. METHODS: To evaluate the effects of hydrogen peroxide on keratinocyte viability, migration and proliferation, sulforhodamine B test, migration assay, and thymidine incorporation assay were performed, respectively. Diluted solutions of 30% hydrogen peroxide( X 50, X 100, X 500, X 1000) were used as testing material and growth medium alone as a control.
