Dietary Oleate Has Beneficial Effects on Every Step of Non-Alcoholic Fatty Liver Disease Progression in a Methionine- and Choline-Deficient Diet-Fed Animal Model.
10.4093/dmj.2011.35.5.489
- Author:
Ji Young LEE
1
;
Jae Hoon MOON
;
Jong Suk PARK
;
Byung Wan LEE
;
Eun Seok KANG
;
Chul Woo AHN
;
Hyun Chul LEE
;
Bong Soo CHA
Author Information
1. Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. bscha@yuhs.ac
- Publication Type:Original Article
- Keywords:
ChREBP;
Fatty acids, monounsaturated;
Non-alcoholic steatohepatitis
- MeSH:
Alanine Transaminase;
Animals;
Aspartate Aminotransferases;
Chemokine CCL2;
Corneal Dystrophies, Hereditary;
Diet;
Disease Progression;
Fatty Acids, Monounsaturated;
Fatty Liver;
Fibrosis;
Inflammation;
Liver;
Matrix Metalloproteinase 9;
Mice;
Models, Animal;
Oleic Acid;
RNA, Messenger;
Tumor Necrosis Factor-alpha
- From:Diabetes & Metabolism Journal
2011;35(5):489-496
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is increasingly recognized as a major cause of liver-related morbidity and mortality. The underlying mechanisms of disease progression remain poorly understood, and primary therapy of NAFLD is not yet established. We investigated the effects of dietary oleate on the development and progression of NAFLD in a methionine- and choline-deficient (MCD) diet-fed animal model. METHODS: A total of 30 C57BL/6J mice were randomly divided into three groups (n=10 in each group) and fed various experimental diets for four weeks: chow, MCD diet, or OMCD (MCD diet with oleate, 0.5 mg/g/day). Liver samples were examined for steatohepatitis and fibrosis parameters and associated genes. RESULTS: Additional dietary oleate dramatically reduced MCD diet-induced hepatic steatosis. Hepatic carbohydrate responsive element-binding protein was overexpressed in MCD diet-fed mice, and dietary oleate prevented this overexpression (P<0.001). Dietary oleate partially prevented MCD diet-induced serum level increases in aspartate aminotransferase and alanine aminotransferase (P<0.001, respectively). The mRNA expressions of hepatic monocyte chemoattractant protein 1, tumor necrosis factor-alpha and matrix metalloproteinase-9 were increased in MCD diet-fed mice, and this overexpression of inflammatory molecules was prevented by dietary oleate (P<0.001). Hepatic pericellular fibrosis was observed in MCD diet-fed mice, and dietary oleate prevented this fibrosis. Altogether, dietary oleate prevented MCD diet-induced hepatic steatosis, inflammation and fibrosis. CONCLUSION: Dietary oleate has beneficial effects in every step of NAFLD development and progression and could be a nutritional option for NAFLD prevention and treatment.
