Plasticity of Cloned Human Marrow Adipocytes between Osteogenesis and Adipogenesis.
- Author:
So Ra PARK
1
Author Information
1. Department of Physiology, Inha University, Inchon, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Human;
Bone marrow;
Adipocyte;
Osteoblast;
Osteogenesis;
Adipogenesis
- MeSH:
Adipocytes*;
Adipogenesis*;
Alkaline Phosphatase;
Bone Marrow*;
Clone Cells*;
Humans*;
Immunohistochemistry;
Mesenchymal Stromal Cells;
Metabolic Diseases;
Osteoblasts;
Osteocalcin;
Osteogenesis*;
Osteoporosis;
Phenotype;
Plastics*
- From:Journal of Korean Orthopaedic Research Society
1998;1(2):239-247
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Information on the interconversion potential of adipocytes, the key in the understanding of bone turnover in metabolic diseases such as osteoporosis has been limited by the lack of pure adipocytic cell population. The object of the present study was i) to isolate pure populations of adipocytes from human bone marrow, ii) to clone single adipocytes from these populations, and iii) to examine in vitro the interconversion potential of the progeny of these single cloned adipocytes between the osteogenic and adipogenic phenotypes. Adipogenic colonies were isolated from the low-density floating fraction of normal bone marrow cultured in adipogenic media for 4 days. Single adipocytes were isolated and cloned by limiting dilution. The cloned adipocytes were found to dedifferentiate into fibroblastlike cells, and subsequently to differentiate into two morphologically distinct cell types: osteoblasts and adipocytes in appropriate culture systems. The adipocytic phenotype was confirmed by morphology, oil red O staining and immunocytochemistry using antiserum to aP2. The osteogenic phenotype was confirmed by alkaline phosphatase, osteocalcin immunostaing using an osteocalcin antiserum OS35 and mineralized nodule formation. These findings confirm that there is plasticity between the differentiation of adipocytic and osteogenic cells in human bone marrow stromal cell cultures. In conclusion, we have shown the ability of isolated clonal adipogenic cells to redifferentiate into cells of the osteogenic and adipogenic lineage and defined the conditions required for the in vitro modulation of adipogenic differentiation. These results provide further evidence that the osteogenic and adipogenic cells share a common multipotential precursor.
