Evaluation of Rapid Diagnostics for Plasmodium falciparum and P. vivax in Mae Sot Malaria Endemic Area, Thailand.
- Author:
Wanna CHAIJAROENKUL
1
;
Thanee WONGCHAI
;
Ronnatrai RUANGWEERAYUT
;
Kesara NA-BANGCHANG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Plasmodium falciparum; Plasmodium vivax; malaria diagnosis; rapid detection test (RDT); Thailand
- MeSH: Antigens, Protozoan/blood; Cross-Sectional Studies; *Diagnostic Techniques and Procedures/instrumentation; Endemic Diseases/statistics & numerical data; Humans; Malaria/*diagnosis/epidemiology/parasitology; Malaria, Vivax; Plasmodium falciparum/genetics/immunology/*isolation & purification; Reagent Kits, Diagnostic; Thailand/epidemiology
- From:The Korean Journal of Parasitology 2011;49(1):33-38
- CountryRepublic of Korea
- Language:English
- Abstract: Prompt and accurate diagnosis of malaria is the key to prevent disease morbidity and mortality. This study was carried out to evaluate diagnostic performance of 3 commercial rapid detection tests (RDTs), i.e., Malaria Antigen Pf/Pantrade mark, Malaria Ag-Pftrade mark, and Malaria Ag-Pvtrade mark tests, in comparison with the microscopic and PCR methods. A total of 460 blood samples microscopically positive for Plasmodium falciparum (211 samples), P. vivax (218), mixed with P. falciparum and P. vivax (30), or P. ovale (1), and 124 samples of healthy subjects or patients with other fever-related infections, were collected. The sensitivities of Malaria Ag-Pftrade mark and Malaria Antigen Pf/Pantrade mark compared with the microscopic method for P. falciparum or P. vivax detection were 97.6% and 99.0%, or 98.6% and 99.0%, respectively. The specificities of Malaria Ag-Pftrade mark, Malaria Ag-Pvtrade mark, and Malaria Antigen Pf/Pantrade mark were 93.3%, 98.8%, and 94.4%, respectively. The sensitivities of Malaria Ag-Pftrade mark, Malaria Antigen Pf/Pantrade mark, and microscopic method, when PCR was used as a reference method for P. falciparum or P. vivax detection were 91.8%, 100%, and 96.7%, or 91.9%, 92.6%, and 97.3%, respectively. The specificities of Malaria Ag-Pftrade mark, Malaria Ag-Pvtrade mark, Malaria Antigen Pf/Pantrade mark, and microscopic method were 66.2%, 92.7%, 73.9%, and 78.2%, respectively. Results indicated that the diagnostic performances of all the commercial RDTs are satisfactory for application to malaria diagnosis.
