Influencing Factors for Cure of Clonorchiasis by Praziquantel Therapy: Infection Burden and CYP3A5 Gene Polymorphism.
- Author:
Chung Hyeon KIM
1
;
Jeong Keun LEE
;
Byung Suk CHUNG
;
Shunyu LI
;
Min Ho CHOI
;
Sung Tae HONG
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: Clonorchis sinensis; clonorchiasis; praziquantel; cyochrome P450; CYP3A5; single nucleotide polymorphism (SNP); EPG
- MeSH: Adolescent; Adult; Aged; Aged, 80 and over; Animals; Child; Clonorchiasis/*drug therapy/*genetics/parasitology; Clonorchis sinensis/isolation & purification/physiology; Cytochrome P-450 CYP3A/*genetics; Feces/parasitology; Female; Humans; Male; Middle Aged; *Polymorphism, Single Nucleotide; Praziquantel/*therapeutic use; Treatment Outcome; Young Adult
- From:The Korean Journal of Parasitology 2011;49(1):45-49
- CountryRepublic of Korea
- Language:English
- Abstract: Chemotherapy of clonorchiasis with praziquantel (PZQ) is effective but about 15% of treated cases have been reported uncured. The present study investigated correlation of single nucleotide polymorphisms (SNPs) of the cytochrome P450 gene, CYP3A5 and cure of clonorchiasis. A total of 346 egg passing residents were subjected and treated by 3 doses of 25 mg/kg PZQ. Reexamination recognized 33 (9.5%) uncured and 313 cured. Numbers of eggs per gram of feces (EPGs) before treatment were significantly lower in the cured group than in the uncured group (2,011.2+/-3,600.0 vs 4,998.5+/-7,012.0, P<0.001). DNAs of the subjects were screened for SNPs at 7 locations of CYP3A5 using PCR. In the uncured group, the SNP frequencies at g.-20555G>A and g.27526C>T of CYP3A5 were 15.2% and 9.1% while those were 3.8% and 1.0%, respectively, in the cured group. The cure rate was significantly lower in the cases with SNP at g.27526C>T and EPGs> or =1,000. In conclusion, EPGs and SNPs of CYP3A5 are factors which influence cure of clonorchiasis by PZQ therapy. It is strongly suggested to recommend 2-day medication for individuals with high EPGs> or =1,000.
