CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax.
- Author:
Hyo Jin KIM
1
;
Bong Kwang JUNG
;
Jin Joo LEE
;
Kyoung Ho PYO
;
Tae Yun KIM
;
Byung il CHOI
;
Tae Woo KIM
;
Hajime HISAEDA
;
Kunisuke HIMENO
;
Eun Hee SHIN
;
Jong Yil CHAI
Author Information
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords: Plasmodium vivax; DNA vaccine; apical membrane antigen (AMA); CD8+ T-cell; gene gun
- MeSH: Animals; Antigens, Protozoan/administration & dosage/genetics/*immunology; CD8-Positive T-Lymphocytes/*immunology; COS Cells; Cercopithecus aethiops; Humans; Lymphocyte Activation; Malaria, Vivax/*immunology/parasitology; Membrane Proteins/administration & dosage/genetics/*immunology; Mice; Mice, Inbred BALB C; Plasmodium vivax/genetics/*immunology; Protozoan Proteins/administration & dosage/genetics/*immunology; Protozoan Vaccines/administration & dosage/genetics/*immunology; Vaccines, DNA/administration & dosage/genetics/*immunology
- From:The Korean Journal of Parasitology 2011;49(1):85-90
- CountryRepublic of Korea
- Language:English
- Abstract: Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.
