Regulation of Inflammatory Response in Periodontal Ligament Cells by Transglutaminase 2.
10.11620/IJOB.2017.42.4.191
- Author:
Sun Young LEE
1
;
Cheol Hun JANG
;
Je Hwang RYU
Author Information
1. Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea. jesryu@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
Periodontal ligament cells;
Transglutaminase 2;
Inflammation;
Periodontitis
- MeSH:
Apoptosis;
Calcium;
Cyclooxygenase 2;
Cytokines;
Extracellular Matrix;
Inflammation;
Interleukin-6;
Necrosis;
Periodontal Ligament*;
Periodontitis;
RNA, Messenger
- From:International Journal of Oral Biology
2017;42(4):191-196
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Transglutaminase2 (TGM2) is a multi-functional calcium dependent enzyme that affects angiogenesis, apoptosis, differentiation, attachment, and changes in the extracellular matrix. However, its function in periodontal tissue has not yet been studied. The aim of this study was to investigate the association of the TGM2 expression and the modulation of inflammatory mediators in inflamed periodontal ligament (PDL) cells induced by pro-inflammatory cytokines such as Interleukin-1β and the Tumor necrosis factor-α. The expression of TGM2 was increased in the inflamed periodontal tissue and PDL cells. Over-expressed TGM2 in the PDL cells increased expression of MMP1, MMP3, IL-6, CXCL8, and PTGS2. Conversely, inhibition of TGM2 activity using LDN27219, a TGM2 inhibitor, resulted in decreased expression of MMP1, MMP3, IL-6, and CXCL8. The mRNA expression was confirmed by RT-PCR and quantified by qRT-PCR. Protein levels were also confirmed by immunofluoroscence staining. These results suggest that TGM2 plays an important role in the regulation of inflammatory mediators which exacerbate tissue damage in inflamed periodontal tissue.
