Capillary Electrophoresis Detection of Hydrogen Peroxide by Using Titanium Ion and 4-(2-thiazolylazo)resorcinol.
10.11620/IJOB.2017.42.4.197
- Author:
Dong Vu PHUONG
1
;
Hoon YOO
Author Information
1. Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University, Gwangju 501-759 South Korea. hoon_yoo@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Hydrogen peroxide;
capillary electrophoresis (CE);
titanium ion (Ti³⁺);
4-(2-thiazolylazo)resorcinol (TAR)
- MeSH:
Capillaries*;
Coffee;
Electrophoresis, Capillary*;
Hydrogen Peroxide*;
Hydrogen*;
Limit of Detection;
Methods;
Tea;
Titanium*
- From:International Journal of Oral Biology
2017;42(4):197-201
- CountryRepublic of Korea
- Language:English
-
Abstract:
A novel method for the detection of hydrogen peroxide in aqueous solution was developed via reaction between H₂O₂, trivalent titanium ion (Ti³⁺) and 4-(2-thiazolylazo) resorcinol (TAR), resulting in a ternary complex with a maximum UV absorbance at 530 nm. The CE detection of H₂O₂ was fast, sensitive and cost-effective without pretreatment procedures. H₂O₂ was detected within 15 min at 1 to 100 µM range with the lowest detection limit at 1.0 µM. Under the optimized CE conditions, the concentration of H₂O₂ in coffee or tea extract was quantitatively determined. Our results show that CE detection of the ternary complex of H₂O₂-Ti³⁺-TAR has potential applications for the detection of H2O2 in aqueous sources.
