Growth and Characterization of the Uveal Melanoma in Vitro.
- Author:
Myung Kyoo KO
1
;
Joon Kiu CHOE
;
Dong Seob KIM
Author Information
1. Department of Ophthalmology, School of Medicine, Hanyang University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
feeder layer;
fibroblast;
melanoma culture;
Vero cell
- MeSH:
Choroid;
Feeder Cells;
Fibroblasts;
Humans;
Melanoma*;
Microscopy, Phase-Contrast;
Vero Cells
- From:Journal of the Korean Ophthalmological Society
1990;31(3):303-308
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed to observe the influence of the feeder layer on the culture of the human uveal melanoma cell. Two types of melanoma cells which were obtained from the patients with choroidal melanoma were implanted into the culture flask. The melanoma cell was seeded onto the culture dish covered with conjunctival fibroblast, Vero cell, and culture dish which did not contain the feeder layer respectively. The growth pattern of the melanoma cell was observed with phase contrast microscopy upto 30 days after seeding. There was no definite difference in growth pattern between each group. These results may indicate that the feeder layer is not an essential factor in the culture of uveal melanoma cell.
