Alteration of CD4+CD25+Foxp3+ T cell level in Kawasaki disease.
10.3345/kjp.2011.54.4.157
- Author:
Su Ye SOHN
1
;
Young Wooh SONG
;
Yun Ku YEO
;
Yun Kyung KIM
;
Gi Young JANG
;
Chan Wook WOO
;
Jung Hwa LEE
;
Kwang Chul LEE
Author Information
1. Department of Pediatrics, Korea University College of Medicine, Seoul, Korea. leejmd@chol.com
- Publication Type:Original Article
- Keywords:
Kawasaki disease;
Regulatory T cell;
CD25+;
Foxp3+
- MeSH:
Flow Cytometry;
Humans;
Immunoglobulins;
Immunoglobulins, Intravenous;
Mucocutaneous Lymph Node Syndrome;
T-Lymphocytes
- From:Korean Journal of Pediatrics
2011;54(4):157-162
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Exaggerated pro-inflammatory reactions during the acute phase of Kawasaki disease (KD) suggest the role of immune dysregulation in the pathogenesis of KD. We investigated the profiles of T regulatory cells and their correlation with the clinical course of KD. METHODS: Peripheral blood mononuclear cells were collected from 17 KD patients during acute febrile and subacute afebrile phases. T cells expressing CD4, CD25, and Foxp3 were analyzed using flow cytometry, and the results were correlated with the clinical course of KD. RESULTS: The percentage of circulating CD4+CD25highFoxp3+ T cells among CD4+ T cells was significantly higher during the subacute afebrile phase than during the acute febrile phase (1.10%+/-1.22% vs. 0.55%+/-0.53%, P=0.049). Although levels of CD4+CD25lowFoxp3+ T cells and CD4+CD25-Foxp3+ T cells were only slightly altered, the percentage of CD4+CD25+Foxp3- T cells among CD4+ T cells was significantly lower during the subacute afebrile phase than during the acute febrile phase (2.96%+/-1.95% vs. 5.64%+/-5.69%, P=0.036). Consequently, the ratio of CD25highFoxp3+ T cells to CD25+Foxp3- T cells was higher during the subacute afebrile phase than during the acute febrile phase (0.45%+/-0.57% vs. 0.13%+/-0.13%, P=0.038). CONCLUSION: Decreased CD4+CD25highFoxp3+ T cells and/or an imbalanced ratio of CD4+CD25highFoxp3+ T cells to CD4+CD25+Foxp3- T cells might play a role in KD development. Considering that all KD patients were treated with intravenous immunoglobulin (IVIG), recovery of CD4+CD25highFoxp3+ T cells during the subacute afebrile phase could be a mechanism of IVIG.
