Immunomodulatory Effects of ZYM-201 on LPS-stimulated B Cells.
- Author:
Ye Eun LEE
1
;
Soochan KIM
;
Woong Jae JUNG
;
Hyung Soo LEE
;
Mi Yeon KIM
Author Information
- Publication Type:In Vitro ; Brief Communication
- Keywords: ZYM-201; B cell; Inflammation; LPS
- MeSH: B-Lymphocytes*; Cell Count; Inflammation; Metabolic Diseases; NF-kappa B; Phosphorylation; Sanguisorba
- From:Immune Network 2014;14(5):260-264
- CountryRepublic of Korea
- Language:English
- Abstract: ZYM-201 is a methyl ester of triterpenoid glycoside from Sanguisorba officinalis which has been used for treatment of inflammatory and metabolic diseases. In this study, immunomodulatory effects of ZYM-201 on B cells were examined in vitro and in vivo. When splenocytes were activated with lipopolysaccharide (LPS), the major population which had shown an increase in cell numbers was B cells. However, when the B cells were treated with ZYM-201 after LPS activation, their cell numbers and the expression of major costimulatory molecules, CD80 and CD86, were decreased. Furthermore, the effect of LPS, which induces activation of NF-kappaB, was abolished by ZYM-201: LPS-stimulated B cells showed decrease of phosphorylation after treatment of ZYM-201. The same results were shown in vivo experiments. These results suggest that ZYM-201 may play a role in the modulation of inflammatory responses through inhibiting NF-kappaB activation and downregulating the expression of costimulatory molecules on B cells.
