Application of Diagnostic Microarray Technique in Subtyping and Pathotyping of Avian Influenza Viruses Isolated in Mongolia.
- Author:
Jung Hoon KWON
1
;
Ji Hoon KIM
;
Dong hun LEE
;
Hyunseok CHO
;
Seung Yong HWANG
;
Seong Su YUK
;
Tseren Ochir ERDENE-OCHIR
;
Jin Yong NOH
;
Woo Tack HONG
;
Jei Hyun JEONG
;
Sol JEONG
;
Gyeong Bin GWON
;
Sang Won LEE
;
In Soo CHOI
;
Chang Seon SONG
Author Information
- Publication Type:Original Article
- Keywords: Influenza virus; HPAI; Microarray; Mongolia; Rapid diagnosis
- MeSH: Animals; Birds; Disease Outbreaks; Influenza in Birds*; Mongolia*; Orthomyxoviridae; Poultry
- From:Journal of Bacteriology and Virology 2016;46(1):22-26
- CountryRepublic of Korea
- Language:Korean
- Abstract: Asian-lineage H5 highly pathogenic avian influenza (HPAI) viruses have caused continuous outbreaks in poultry and wild birds. Development of rapid and accurate diagnostic methods is needed for preventing further spread of the virus and reducing the time required for eradication of the virus. We developed a low-density microarray for the rapid detection and identification of avian influenza virus subtypes H5, H7, and H9 and their pathotypes in a previous study. In the present study, we evaluated previously developed diagnostic microarray using avian influenza viruses isolated in Mongolia, including H5 HPAI viruses. All H5 HPAI viruses isolated in Mongolia were shown as H5-specific and highly pathogenic pattern in the microarray. H2, H3 and H12 viruses isolated in Mongolia used in this study did not show any H5, H7 and H9 patterns. These results indicated that this diagnostic microarray has enormous potential for the rapid subtyping and pathotyping of influenza viruses, including viruses isolated in Mongolia.
