Diagnosis of Lymphoid Malignancy by PCR for Analysis of Antigen Receptor Rearrangement after Blood Transfusion in a Dog with Acute Lymphocytic Leukemia.
- Author:
Suhee KIM
1
;
Hyunwoo KIM
;
Soo Hyeon LEE
;
Ilhan CHO
;
Seongwoo KANG
;
Junwoo BAE
;
Woosun KIM
;
Soomin AHN
;
Jihye CHOI
;
Sang Ki KIM
;
Yoonjung DO
;
Jae Gyu YOO
;
Jinho PARK
;
DoHyeon YU
Author Information
- Publication Type:Case Report
- Keywords: Acute lymphoblastic leukemia; PARR; Flow cytometry; Blood transfusion; Lymphoid malignancy
- MeSH: Adolescent; Anemia; Animals; Blood Cell Count; Blood Transfusion*; Diagnosis*; Dogs*; Female; Flow Cytometry; Hospitals, Teaching; Humans; Lethargy; Leukopenia; Lymphocyte Subsets; Methods; Mucous Membrane; Polymerase Chain Reaction*; Precursor Cell Lymphoblastic Leukemia-Lymphoma*; Receptors, Antigen*; T-Lymphocytes; Thrombocytopenia
- From:Immune Network 2017;17(4):269-274
- CountryRepublic of Korea
- Language:English
- Abstract: Acute lymphocytic leukemia (ALL) is uncommon lymphoid malignancy in dogs, and its diagnosis is challenging. A 14-year-old spayed female mixed breed dog was transferred to a veterinary medical teaching hospital for an immediate blood transfusion. The dog showed lethargy, pale mucous membranes, and a weak femoral pulse. Complete blood count revealed non-regenerative anemia and severe leukopenia with thrombocytopenia. ALL was tentatively diagnosed based on the predominance of immature lymphoblasts on blood film examination. For confirmation of lymphoid malignancy, PCR for antigen receptor rearrangement (PARR) on a peripheral blood sample and flow cytometry analysis were performed after blood transfusion. Flow cytometry analysis revealed that lymphocyte subsets were of normal composition, but PARR detected a T-cell malignancy. The dog was diagnosed with ALL and survived 1 wk after diagnosis. In conclusion, after blood transfusion, flow cytometry was not a reliable diagnostic method for an ALL dog, whereas PARR could detect lymphoid malignancy. Our results suggest that PARR should be the first-line diagnostic tool to detect canine lymphoid malignancy after a blood transfusion.
