Role of multicolor fluorescence in situ hybridization (FISH) in simultaneous detection of probe sets for chromosome 18, X and Y in uncultured amniotic fluid cells.
10.3346/jkms.1999.14.4.438
- Author:
Jong In KIM
1
;
Jeong Ho RHEE
Author Information
1. Department of Obstetrics and Gynecology, Keimyung University, School of Medicine, Taegu, Korea. k1011@dsmc.or.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Prenatal diagnosis;
In situ hybridization, fluorescence;
Amniotic fluid
- MeSH:
Amniocentesis/methods*;
Amniotic Fluid/cytology;
Aneuploidy;
Centromere/genetics;
Chromosomes, Human, Pair 18*;
Color;
DNA Probes;
DNA, Satellite/analysis;
Female;
Human;
In Situ Hybridization, Fluorescence/methods*;
Karyotyping;
Pregnancy;
Sex Chromosome Abnormalities/genetics;
Sex Chromosome Abnormalities/diagnosis*;
X Chromosome*;
Y Chromosome*
- From:Journal of Korean Medical Science
1999;14(4):438-442
- CountryRepublic of Korea
- Language:English
-
Abstract:
Major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid analysis of chromosome copy number in interphase cells. The purpose of this study was to evaluate the role of multicolor fluorescence in situ hybridization in simultaneous detection of probe sets for chromosome 18, X, and Y in uncultured amniotic fluid cells as a safer alternative method for aneuploidy detection prenatally. Fifty amniotic fluid samples were analyzed by FISH and standard cytogenetics. Mean time to obtain results was three days for fluorescence in situ hybridization and 20 days for karyotype. Fluorescence in situ hybridization was informative in 43 samples (86%), and within this group, two aneuploidies were correctly identified. This evaluation demonstrates that FISH with X, Y, and 18 alpha satellite DNA probes could accurately and rapidly detect aneuploidies involving these chromosomes and could be used in any prenatal clinical laboratory.
