Production of Stromal Cell-Derived Factor-1 (SDF-1)and Expression of CXCR4 in Human Bone Marrow Endothelial Cells.
10.3346/jkms.2003.18.5.679
- Author:
Hwan Jung YUN
1
;
Deog Yeon JO
Author Information
1. Division of Hematology/Oncology, Department of Internal Medicine, College of Medicine, Chungnam National University, Daejon, Korea. deogyeon@cnu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Bone Marrow;
Endothelium, Vascular;
Chemokines, CXC;
Receptors, CXCR4;
Chemotaxis
- MeSH:
Antigens, CD34/biosynthesis;
Bone Marrow Cells/*metabolism;
Cell Movement;
Cells, Cultured;
Chemokines, CXC/*biosynthesis;
Chemotaxis;
Culture Media, Conditioned/pharmacology;
Endothelial Cells/*metabolism;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Hematopoietic Stem Cells/metabolism;
Human;
Immunohistochemistry;
Pertussis Toxin/pharmacology;
RNA, Messenger/metabolism;
Receptors, CXCR4/*biosynthesis;
Reverse Transcriptase Polymerase Chain Reaction;
Support, Non-U.S. Gov't;
Time Factors;
Umbilical Veins/cytology
- From:Journal of Korean Medical Science
2003;18(5):679-685
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated the production of stromal cell-derived factor-1 (SDF-1) and the expression of CXCR4 in human bone marrow endothelial cells (BMECs). Human BMEC cell line BMEC-1 cells expressed SDF-1 mRNA, and conditioned medium induced chemoattraction of CD34+ cells. Migration was not inhibited by pretreating the input cells with pertussis toxin, indicating that the chemoattractive activity was not dependent on SDF-1. Three-day culture of BMEC-1 and primary human BMEC cells produced 1,710+/-204 and 1,050+/-153 pg/mL SDF-1alpha, respectively, which was much less than primary human BM stromal cells (29,536+/-532 pg/ mL). By immuno-histochemistry, CXCR4 was detected in the endothelial cells lining sinusoids, arterioles, and venules in the bone marrow. However, cultured BMECs and BMEC-1 cells did not express CXCR4 on their surfaces. These results indicate that BMECs produce and release small amounts of SDF-1 and express CXCR4 in vivo only.
