Rapid Detection of Duplication/Deletion of the PMP22 Gene in Patients with Charcot-Marie-Tooth Disease Type 1A and Hereditary Neuropathy with Liability to Pressure Palsy by Real-time Quantitative PCR using SYBR Green I Dye.
10.3346/jkms.2003.18.5.727
- Author:
Sang Wun KIM
1
;
Kwang Soo LEE
;
Hyun Seok JIN
;
Tae Mi LEE
;
Soo Kyung KOO
;
Yong Jun LEE
;
Sung Chul JUNG
Author Information
1. Division of Genetic Disease, Department of Biomedical Science, National Institute of Health, Seoul, Korea. scjung@nih.go.kr
- Publication Type:Original Article
- Keywords:
Charcot-Marie-Tooth disease;
Hereditary neuropathy with liability to pressure palsy;
Reactions, polymerase chain SYBR Green
- MeSH:
Charcot-Marie-Tooth Disease/*diagnosis/*genetics;
Chromosomes, Human, Pair 17;
Family Health;
Female;
Fluorescent Dyes/*pharmacology;
Gene Deletion;
Gene Duplication;
Hereditary Motor and Sensory Neuropathies/*genetics;
Human;
Male;
Membrane Proteins/*biosynthesis;
Organic Chemicals/*pharmacology;
Paralysis/*genetics;
Peripheral Nervous System Diseases/*genetics;
Reverse Transcriptase Polymerase Chain Reaction
- From:Journal of Korean Medical Science
2003;18(5):727-732
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mutations and altered gene dosage of the peripheral myelin protein (PMP22) gene in chromosome 17p11.2-12 are the main causes for hereditary neuropathies, accounting for approximately 70% of all cases. Patients with duplication of the PMP22 develop Charcot-Marie-Tooth disease type 1A (CMT1A) and deletion of one PMP22 allele leads to hereditary neuropathy with liability to pressure palsy (HNPP). Twenty patients with CMT1A, 17 patients with HNPP, and 18 normal family members and 28 normal controls were studied by real-time quantitative PCR using SYBR Green I on the ABI 7700 Sequence Detection System. The copy number of the PMP22 gene was determined by the comparative threshold cycle method and the albumin was used as a reference gene. The PMP22 duplication ratio ranged from 1.45 to 2.06 and the PMP22 deletion ratio ranged from 0.42 to 0.64. The PMP22 ratio in normal controls, including normal family members, ranged from 0.85 to 1.26. No overlap was found between patients with CMT1A or patients with HNPP and normal controls. This method is fast, highly sensitive, specific, and reproducible in detecting PMP22 duplication and deletion in CMT1A and HNPP patients, respectively.
