Diallyl disulfide attenuates acetaminophen-induced renal injury in rats.
10.5625/lar.2016.32.4.200
- Author:
Jin Young SHIN
1
;
Ji Hee HAN
;
Je Won KO
;
Sung Hyeuk PARK
;
Na Rae SHIN
;
Tae Yang JUNG
;
Hyun A KIM
;
Sung Hwan KIM
;
In Sik SHIN
;
Jong Choon KIM
Author Information
1. Ministry of Food and Drug Safety, Cheongju, Korea.
- Publication Type:Original Article
- Keywords:
Acetaminophen;
nephrotoxicity;
KIM-1;
NGAL;
diallyl disulfide;
protective effect
- MeSH:
Acetaminophen;
Acute Kidney Injury;
Animals;
Biomarkers;
Blood Urea Nitrogen;
Blotting, Western;
Humans;
Immunohistochemistry;
Kidney;
Lipocalins;
Male;
Neutrophils;
Rats*
- From:Laboratory Animal Research
2016;32(4):200-207
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study investigated the protective effects of diallyl disulfide (DADS) against acetaminophen (AAP)-induced acute renal injury in male rats. We also investigated the effects of DADS on kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL), which are novel biomarkers of nephrotoxicity in renal tissues, in response to AAP treatment. The following four experimental groups were evaluated: (1) vehicle control, (2) AAP (1,000 mg/kg), (3) AAP&DADS, and (4) DADS (50 mg/kg/day). AAP treatment caused acute kidney injury evidenced by increased serum blood urea nitrogen (BUN) levels and histopathological alterations. Additionally, Western blot and immunohistochemistry analysis showed increased expression of KIM-1 and NGAL proteins in renal tissues of AAP-treated rats. In contrast, DADS pretreatment significantly attenuated the AAP-induced nephrotoxic effects, including serum BUN level and expression of KIM-1 and NGAL proteins. Histopathological studies confirmed the renoprotective effect of DADS. The results suggest that DADS prevents AAP-induced acute nephrotoxicity, and that KIM-1 and NGAL may be useful biomarkers for the detection and monitoring of acute kidney injury associated with AAP exposure.
