Effects of irradiation on the mRNA expression of the osteocalcin and osteopontin in MC3T3-E1 osteoblastic cell line.
- Author:
Su Beom CHO
1
;
Ssang Yong HA
;
Sang Rae LEE
;
Kwang Joon KOH
Author Information
1. Department of Oral and Maxillofacial Radiology, School of Dentistry, Institute of Oral Bio Science, Chonbuk National University. radkoh@moak.chonbuk.ac.kr
- Publication Type:Original Article
- Keywords:
MC3T3-E1 osteoblastic cell line;
radiation;
osteocalcin;
osteopontin
- MeSH:
Cell Line*;
Cesium;
DNA, Complementary;
Osteoblasts*;
Osteocalcin*;
Osteogenesis;
Osteopontin*;
Polymerase Chain Reaction;
RNA;
RNA, Messenger*
- From:Korean Journal of Oral and Maxillofacial Radiology
2003;33(3):179-185
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-E1 osteoblastic cell line, particularly on the expression of osteocalcin and osteopontin. MATERIALS AND METHODS: Cells were irradiated with a single dose of 0.5, 1, 4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. After the specimens were harvested, RNA was extracted on the 3rd, 7th, 14th, and 21st day after irradiation. The RNA strands were reverse-transcribed and the resulting cDNAs were subjected to amplification by PCR. RESULTS: The irradiated cells demonstrated a dose-dependent increase in osteocalcin and a dose-dependent decrease in osteopontin mRNA expression compared with the non-irradiated control group. The amount of osteocalcin mRNA expression decreased significantly at the 3rd day after irradiation of 0.5, 1, 4, and 8 Gy, and also decreased significantly at the 3rd, 14th, and 21st day after irradiation in the 8 Gy exposed group compared with the control group. The degree of osteopontin mRNA expression increased significantly at the 7th day after irradiation of 0.5, 1, 4, and 8 Gy. CONCLUSION: These results showed that each single dose of 0.5, 1, 4, and 8 Gy influenced the mRNA expression of osteocalcin and osteopontin associated with the calcification stage of osteoblastic cells, suggesting that each single dose affected bone formation at the cell level.
