Localization of transforming growth factor beta1 in airway mucosa of toluene diisocyanate-induced asthma.
- Author:
Soo Keol LEE
1
;
Sun Min LEE
;
Ki Baik HAHM
;
Hyun Ee YIM
;
Choon Sik PARK
;
Sun Sin KIM
;
Dong Ho NAHM
;
Hae Sim PARK
Author Information
1. Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon. hspark@madang.ajou.ac.kr
- Publication Type:Original Article
- Keywords:
Localization of TGF beta1;
bronchial mucosa;
TDI-induced asthma
- MeSH:
Asthma*;
Humans;
Immunohistochemistry;
Lung;
Mucous Membrane*;
Pneumonectomy;
Toluene*;
Transforming Growth Factor beta1*;
Transforming Growth Factors*
- From:Journal of Asthma, Allergy and Clinical Immunology
2001;21(3):500-507
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVE: There have been some reports on the expression of TGF beta-an anti-inflammatory and fibrosing cytokine in airway mucosa of asthmatics. However, the ex- pression of TGF beta1 in bronchial mucosa of TDI-induced asthma is not known. The aim of this study was to observe immunolocalization of TGF beta1 in airway mucosa of TDI-induced asthma. METHODS: TGF beta1 expressions were compared using immunohistochemistry in bronchial muco- sa from 22 subjects with TDI-induced asthma (group I: 10 newly diagnosed, group II: 12 subjects with persistent asthma symptoms for more than 4 years after diagnosis), and 8 non- asthmatics undergoing pneumonectomy from lung tumor. The distribution and intensity of expression were analyzed by two observers in four areas of bronchial tissue-epithelium(EP), vascular endothelium(VE), smooth muscle(SM), and mucous glands(MG). RESULTS: Positive rates of TGF beta1 expression for groups I and II in the four areas were as follows; EP (50% vs 100%, p<0.05), VE(40% vs 100%, p<0.05), SM(33.3% vs 100%, p<0.05), and MG(66.7% vs 80%, p>0.05). Grades of TGF beta1 expression of EP, VE, and SM were significantly higher in group II than in group I(p<0.05, respectively). There was no significant difference in distribution and intensity of TGF beta1 expression between TDI-induced asthma and controls(p>0.05, respectively). CONCLUSION: TGF beta1 expressions of EP, VE, SM and MG were noted in airway mucosa of TDI-induced asthma and expressions of EP, VE, and SM were more intense and frequent in patients with persistent asthma symptoms.
