Simultaneous HPLC Analysis of Three Flavonoids in the Extracts of Artocarpus heterophyllus Heartwoods.
10.20307/nps.2016.22.2.77
- Author:
Abdi Wira SEPTAMA
1
;
Pharkphoom PANICHAYUPAKARANANT
Author Information
1. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. pharkphoom.p@psu.ac.th
- Publication Type:Original Article
- Keywords:
Artocarpus heterophyllus;
Artocarpanone;
Artocarpin;
Cycloartocarpin;
HPLC
- MeSH:
Artocarpus*;
Calibration;
Chromatography, High Pressure Liquid*;
Flavonoids*;
Hot Temperature;
Methanol;
Methods;
Sensitivity and Specificity;
Water
- From:Natural Product Sciences
2016;22(2):77-81
- CountryRepublic of Korea
- Language:English
-
Abstract:
A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of three antibacterial flavonoids, artocarpanone, artocarpin, and cycloartocarpin in ethyl acetate extracts from Artocarpus heterophyllus heartwoods. Separation was achieved using a TSK-gel ODS-80Tm column (5 µm, 4.6 × 150 mm) at 25℃ with a gradient elution system of methanol and water as follows: 0-8 min, 60:40; 8-27 min, 80:20; 27-35 min, 60:40, v/v, at a flow rate of 1 mL/min, and a quantitative UV detection at 285 nm. The method was validated by measuring the key parameters, including specificity, linearity, sensitivity, accuracy, repeatability and reproducibility. A high degree of specificity and sensitivity was achieved. The calibration curves for all three flavonoids showed good linearity with a coefficient of determinations (R²) of ≥ 0.9995. The recoveries of the method were from 98-104%, with good reproducibility and repeatability (RSD values of less than 2%) were also achieved. Ethyl acetate was the best solvent for extraction of these three flavonoids using the heat reflux conditions for 1 h. This optimized sample preparation and HPLC method can be practically used for a routine standardization process of the extracts from the A. heterophyllus heartwoods.
