Tea Flavonoids Induced Differentiation of Peripheral Blood-derived Mononuclear Cells into Peripheral Blood-derived Endothelial Progenitor Cells and Suppressed Intracellular Reactive Oxygen Species Level of Peripheral Blood-derived Endothelial Progenitor C.
10.20307/nps.2016.22.2.87
- Author:
Wahyu WIDOWATI
1
;
Laura WIJAYA
;
Dian Ratih LAKSMITAWATI
;
Rahma Micho WIDYANTO
;
Pande Putu ERAWIJANTARI
;
Nurul FAUZIAH
;
Indra BACHTIAR
;
Ferry SANDRA
Author Information
1. Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia. wahyu_w60@yahoo.com
- Publication Type:Original Article
- Keywords:
Tea flavonoids;
Antioxidant;
Endothelial progenitor cell;
Differentiation;
ROS;
Apoptosis
- MeSH:
Apoptosis;
Atherosclerosis;
Catechin;
Electrocardiography;
Endothelial Cells;
Endothelial Progenitor Cells*;
Flavonoids*;
Hydrogen;
Oxidative Stress;
Reactive Oxygen Species*;
Tea*;
Vascular Endothelial Growth Factor Receptor-2
- From:Natural Product Sciences
2016;22(2):87-92
- CountryRepublic of Korea
- Language:English
-
Abstract:
Endothelial dysfunction in atherosclerosis is associated with increasing oxidative stress that could be reversed by antioxidant. Therefore epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C) of tea flavonoids were investigated for their roles in regenerating endothelial cell. Peripheral blood mononuclear cells (PB-MNCs) were isolated, plated and cultured in medium with/without treatment of EGCG, ECG, EGC and C. Results showed that among all EGCG, ECG, EGC and C concentrations tested, 12.5 µmol/L was not cytotoxic for peripheral blood-derived endothelial progenitor cells (PB-EPCs). Treatment of EGCG, ECG, EGC or C increased the percentages of CD34, CD133, VEGFR-2 expressions and suppressed hydrogen peroxide-induced percentages of reactive oxygen species (ROS) level in PB-EPCs. Taken together, our current results showed that EGCG, ECG, EGC or C of tea flavonoids could induce differentiation of PB-MNCs into PB-EPCs as well as protect PB-EPCs from oxidative damage by suppresing the intracellular ROS levels.
