Aldose Reductase Inhibitory Alkaloids from Corydalis ternata.
10.20307/nps.2016.22.2.102
- Author:
Wonse SEO
1
;
Sang Hoon JUNG
;
Sang Hee SHIM
Author Information
1. School of Biotechnology, Yeungnam University, Gyeongsan 38541, Korea.
- Publication Type:Original Article
- Keywords:
Corydalis ternatac;
Aldose reductase inhibition activity;
Isoquinoline alkaloids
- MeSH:
Aldehyde Reductase*;
Alkaloids*;
Corydalis*;
Diabetes Complications;
Humans;
Methanol
- From:Natural Product Sciences
2016;22(2):102-106
- CountryRepublic of Korea
- Language:English
-
Abstract:
A methanolic extract of Corydalis ternata having aldose reductase inhibitory activity was examined as a possible aldose reductase (ALR2) inhibitor, a key enzyme involved in diabetic complications. Seven alkaloids, tetrahydrocoptisine (1), corydaline (2), tetrahydropalmatine (3), isocorybulbine (4), corybulbine (5), dehydrocorydaline (6), and N-methyltetrahydroberbinium (7) were isolated from CHCl₃ fraction of C. ternata methanol extract. Among them, compounds 1, 5, and 7 exhibited 5.04 ± 1.97%, 5.00 ± 1.26%, and 1.80 ± 2.33% inhibitions, respectively at 40 µM. The activities of the single compounds were not comparable to that of the whole extract, suggesting that the whole combination of each single compound was responsible for the activity of the extract as shown in many cases of natural medicines. Even though this is the second report on aldose reductase inhibition activity of C. ternata, recombinant human aldose reductase was employed in this study unlike in the previous report. Furthermore, the aldose reductase inhibitory activities of isocorybulbine, corybulbine, and N-methyltetrahydroberbinium, to the best of our knowledge, were evaluated for the first time in this study. These results suggest a use of the extract of C. ternata for ameliorating diabetic complications.
