The Application of DNA Chip Technology to Identify Herbal Medicines: an Example from the Family Umbelliferae.
- Author:
Pil Ho KIM
1
;
Jisoo PARK
;
Yeong Shik KIM
;
Youngbae SUH
Author Information
1. Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-742, Korea. ysuh@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Authentication;
DNA chip;
Herbal medicines;
ITS;
Umbelliferae
- MeSH:
Apiaceae*;
DNA*;
Far East;
Humans;
Inflammation;
Neuralgia;
Oligonucleotide Array Sequence Analysis*;
Paralysis;
Rhizome
- From:Natural Product Sciences
2015;21(3):185-191
- CountryRepublic of Korea
- Language:English
-
Abstract:
Comparative molecular analysis has been frequently adopted for the authentication of herbal medicines as well as the identification of botanical origins. Roots and rhizomes of the family Umbelliferae have been used as traditional herbal medicines to relieve various symptoms such as inflammation, neuralgia and paralysis in countries of East Asia. Since most herbal medicines of the Umbelliferae roots or rhizomes are generally supplied in the form of dried slices, morphological examination does not often provide sufficient evidence to identify the botanical origin. Using species-specific probes developed by the comparative analysis of nrDNA ITS sequences, a DNA chip was developed to identify herbal medicines for 13 species in the Umbelliferae. The developed DNA Chip proves its potential as a rapid, sensitive and effective tool for authenticating herbal medicines in future.
