Anti-oxidant and Hepatoprotective Effect of White Ginsengs in H2O2-Treated HepG2 Cells.
- Author:
Shanmugam PARTHASARATHI
1
;
Se Chul HONG
;
Myeong Hwan OH
;
Young Sik PARK
;
Ji Hyun YOO
;
Su Yeon SEOL
;
Hwan LEE
;
Jong Dae PARK
;
Mi Kyung PYO
Author Information
1. International Ginseng & Herb Research Institute, Geumsan 312-804, Korea. pmk67@ginherb.re.kr
- Publication Type:Original Article
- Keywords:
White ginseng;
Antioxidant;
Cell viability;
Chemoprevention
- MeSH:
Catalase;
Cell Survival;
Chemoprevention;
Ethanol;
Functional Food;
Hep G2 Cells*;
Herbal Medicine;
Hydroxyl Radical;
Korea;
Lipid Peroxidation;
Liver;
Panax*;
Superoxide Dismutase;
Water
- From:Natural Product Sciences
2015;21(3):210-218
- CountryRepublic of Korea
- Language:English
-
Abstract:
The antioxidant activity of white ginseng was not recorded in Korea Functional Food Code, while its activity of red ginsengs was recorded. The aim of this study was to evaluate the antioxidant and hepato protective effect of different ginsengs in H2O2-treated HepG2 cells. White and red ginseng were prepared from longitudinal section of the same fresh ginseng (4-year old). The whole parts of white and red ginsengs were separately extracted with 70% ethanol and distilled water respectively, at 70 degrees C to obtain therapeutic ginseng extracts namely, WDH (distilled water extract of white ginseng), WEH (70% ethanol extract of white ginseng), RDH (distilled water extract of red ginseng) and REH (70% ethanol extract of red ginseng). In this work, we have investigated the DPPH, hydroxyl radical, Fe2+-chelating activity, intracellular ROS scavenging capacity and lipid peroxidation of different ginsengs. All these extracts showed a dose dependent free-radical scavenging capacity and a ROS generation as well as lipid peroxidation was significantly reduced by treatment with bioactive extracts of white ginsengs (WDH) than red ginsengs. Additionally, white ginseng extracts (WDH) has dramatically increased intracellular antioxidant enzyme activities like superoxide dismutase and catalase in H2O2-treated HepG2 cells. All these results explain that administration of white ginseng is useful as herbal medicine than red ginseng for chemoprevention of liver damage.
