Effect of storage conditions on prothrombin time, activated partial thromboplastin time and fibrinogen concentration on canine plasma samples.
10.4142/jvs.2010.11.2.121
- Author:
Giuseppe PICCIONE
1
;
Stefania CASELLA
;
Claudia GIANNETTO
;
Elisabetta GIUDICE
Author Information
1. Dipartimento di Scienze Sperimentali e Biotecnologie Applicate, Sezione di Fisiologia Applicata ed Etologia Comparata, Facolta di Medicina Veterinaria, Universita degli Studi di Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy. giuseppe.piccione@unime.it
- Publication Type:Original Article
- Keywords:
thromboplastin time;
dog;
fibrinogen;
prothrombin time;
storage conditions
- MeSH:
Animals;
Blood Preservation/methods/*veterinary;
Dogs/*blood;
Fibrinogen/*analysis;
Partial Thromboplastin Time/*veterinary;
Plasma/chemistry;
Prothrombin Time/*veterinary;
Specimen Handling/methods/*veterinary;
Time Factors
- From:Journal of Veterinary Science
2010;11(2):121-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
The present study was to assess the effect of storage conditions on prothrombin time (PT), activated partial thromboplastin time (aPTT) and fibrinogen concentration in blood samples of healthy dogs. Thirty-five dogs of various breeds were included in the study. Citrated blood samples were obtained and plasma was divided into four aliquots to assess selected clotting parameters by means of a coagulometer. The first aliquot was analysed within 1 h after collection, while the remaining 3 were stored at 8degrees C for 4, 8 and 24 h, respectively. One-way repeated measures analysis of variance documented a significant decreasing effect on PT at 24 h compared to 8 h and on fibrinogen concentration after 8 and 24 h compared to sampling time and at 4 and 24 h compared to 8 h post sampling. In conclusion, the results of this study indicate that only fibrinogen appears prone to significant decrease. In fact, aPTT is not substantially affected by refrigeration for at least 24 h post sampling and PT showed a statistical difference that does not necessary indicate biological significance as the results obtained were within reference intervals for the dog.
