spa typing and enterotoxin gene profile of Staphylococcus aureus isolated from bovine raw milk in Korea.
10.4142/jvs.2010.11.2.125
- Author:
Sun Young HWANG
1
;
Young Kyung PARK
;
Hye Cheong KOO
;
Yong Ho PARK
Author Information
1. Department of Microbiology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. yhp@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
bovine mastitis;
enterotoxin;
mobile genetic elements;
S. aureus;
spa typing
- MeSH:
Animals;
Cattle;
Cluster Analysis;
DNA, Bacterial/chemistry/genetics;
Enterotoxins/chemistry/*genetics;
Female;
Genotype;
Mastitis, Bovine/epidemiology/genetics/*microbiology;
Microsatellite Repeats;
Milk/*microbiology;
Polymerase Chain Reaction/veterinary;
Prevalence;
Republic of Korea/epidemiology;
Staphylococcal Infections/epidemiology/microbiology/*veterinary;
Staphylococcus aureus/classification/genetics/*isolation & purification
- From:Journal of Veterinary Science
2010;11(2):125-131
- CountryRepublic of Korea
- Language:English
-
Abstract:
Staphylococcus aureus is a major etiological pathogen of bovine mastitis, which triggers significant economic losses in dairy herds worldwide. In this study, S. aureus strains isolated from the milk of cows suffering from mastitis in Korea were investigated by spa typing and staphylococcal enterotoxin (SE) gene profiling. Forty-four S. aureus strains were isolated from 26 farms in five provinces. All isolates grouped into five clusters and two singletons based on 14 spa types. Cluster 1 and 2 isolates comprised 38.6% and 36.4% of total isolates, respectively, which were distributed in more than four provinces. SE and SE-like toxin genes were detected in 34 (77.3%) isolates and the most frequently detected SE gene profile was seg, sei, selm, seln, and selo genes (16 isolates, 36.3%), which was comparable to one of the genomic islands, Type I nuSabeta. This is a first report of spa types and the prevalence of the recently described SE and SE-like toxin genes among S. aureus isolates from bovine raw milk in Korea. Two predominant spa groups were distributed widely and recently described SE and SE-like toxin genes were detected frequently.
