Effect of Glutathione on Oxidant-induced Cardiotoxicity.
10.4070/kcj.1997.27.4.433
- Author:
Hyang Suk YOON
1
;
Seung Taeck PARK
;
Ock Kyu PARK
Author Information
1. Department of Pediatrics, Wonkwang University School of Medicine, Iksan, Korea.
- Publication Type:Original Article
- Keywords:
Cultured myocardial cells;
Oxygen radicals;
Cardiotoxicity;
Antioxidant
- MeSH:
Animals;
Antioxidants;
Cell Survival;
Glucose;
Glutathione*;
Rats;
Reactive Oxygen Species
- From:Korean Circulation Journal
1997;27(4):433-437
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In order to elucidate toxic mechanism of the oxygen radicals on cultured rat myocardial cells, cytotoxic effect of oxygen radicals was evaluated by MTT assay. In addition protective effect of glutathione(GSH) on oxidant-induced cardiotoxicity was investigated on these cultures. METHODS: Myocardial cells derived from neonatal rats were cultured for 12 hours in the medium containing various concentrations of glucose oxidase(GO). Cell viability was measured by MTT assay and morphological changes of the myocardial cells were observed by light microscope. RESULTS: GO-mediated oxygen radicals remarkably decreased cell viability of cultured myocardial cells in a dose-and time-dependent manner. And also, GSH blicked GO-induced cardiotoxicity in these cultures. CONCLUSION: These results suggest that the oxygen radicals are tixic and the selective antioxidants such as GSH are effective in blocking against the oxidant-induced cardiotoxicity in cultures of the myocardial cells of neonatsl rats.
