Chemical Components from the Stems of Pueraria lobata and Their Tyrosinase Inhibitory Activity.
10.20307/nps.2016.22.2.111
- Author:
Abubaker M A MORGAN
1
;
Mi Ni JEON
;
Min Hye JEONG
;
Seo Young YANG
;
Young Ho KIM
Author Information
1. College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea. yhk@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Pueraria lobata;
Leguminosae;
Tyrosinase;
Enzymatic activity;
Inhibition
- MeSH:
Agaricales;
Chloroform;
Fabaceae;
Genistein;
Monophenol Monooxygenase*;
Pueraria*
- From:Natural Product Sciences
2016;22(2):111-116
- CountryRepublic of Korea
- Language:English
-
Abstract:
Phytochemical investigation of the stems of Pueraria lobata (Wild) Ohwi (Leguminosae), led to the isolation of eighteen known compounds: β-amyrone (1), (+)-pinoresinol (2), (+)-syringaresinol (3) (+)-syringaresinol-O-β-D-glucoside (4), (+)-lariciresinol (5), (-)-tuberosin (6), naringenin (7), liquiritigenin (8), isoliquiritigenin (9) genistein (10), daidzein (11) daidzin (12) daidzein 4',7-diglucoside (13) 2,4,4'-trihydroxy deoxybenzoin (14), S-(+)-1-hydroxy-3-(4-hydroxyphenyl)-1-(4-hydroxy-2-methoxy-phenyl)propan-2-one (15), methyl 2-O-β-D-glucopyranosylbenzoate (16), pyromeconic acid 3-O-β-D-glucopyranoside 6'-(O-4''-hydroxy-3-methoxybenzoate) (17), and allantion (18). The chemical structures of these compounds were elucidated from spectroscopic data and by comparison of those data with previously published results. The effects of isolated compounds on mushroom tyrosinase enzymatic activity were screened. The results indicated that, chloroform extract of P. lobata stems turned out to be having tyrosinase inhibitory effect, and only compounds 5, 8, 9, and 11 showed enzyme inhibitory activity, with IC₅₀ values of 21.49 ± 4.44, 25.24 ± 6.79, 4.85 ± 2.29, and 17.50 ± 1.29 µM, respectively, in comparison with these of positive control, kojic acid (IC₅₀ 12.28 ± 2.72 µM). The results suggest that P. lobata stems extract as well as its chemical components may represent as potential candidates for tyrosinase inhibitors.
